HPLC COLUMN VOLUME TABLE

			
	COLUMN DIMENSIONS {I.D. x L(mm)}	  VOID VOLUME {ml}	
		  1.0   x   100				0.06
		  1.0   x   150				0.08
		  1.0   x   250				0.14
		  1.0   x   300				0.17
		  2.1   x   100				0.24
		  2.1   x   150				0.37
		  2.1   x   250				0.61
		  2.1   x   300				0.73
		  4.6   x   100				1.16
		  4.6   x   150				1.75
		  4.6   x   250				2.90
		  4.6   x   300				3.49
		  7.8   x   100				3.35
		  7.8   x   150				5.02
		  7.8   x   250				8.36
		  7.8   x   300			       10.04
		 10.0   x   100			        5.50
		 10.0   x   150			        8.25
		 10.0   x   250			       13.75
		 10.0   x   300			       16.49
		 21.0   x   100			       24.25
		 21.0   x   150			       36.37
		 21.0   x   250			       60.61
		 21.0   x   300			       72.74
		 50.0   x   100			      137.45
		 50.0   x   150			      206.17
		 50.0   x   250			      343.61
		 50.0   x   300		              412.33
   Void Volume (ul) = (d^2 *Pi * L * Pore Volume) / 4
		             (Note: Column Diameter and Length are in mm)
            * Notes : Minimize all extra-column volume when testing. Assumes an Average Pore Volume of 0.70 (70%) on a bare, fully porous, spherical silica support. 
  • Superficially porous particles (e.g. Fused-Core, Core-Shell) have less volume than the standard fully porous supports (e.g. 50%). 
  • Depending on any endcapping chemistries used (e.g. C8 - C18) the calculated value can differ from the measured value. 
  • Always inject an unretained compound onto your specific column to determine the actual column void volume. 
  • For many common RP columns run with at least 20% organic mobile phase content, compounds such as: Uracil or Thiourea may be tried, but 
some inorganic salts (e.g. sodium nitrite and sodium nitrate) have also been shown to work as well.
  • For many normal phase silica, cellulose, amylose or brush-type supports, 1,3,5-Tri-t-butyl benzene (TTBB) has been used with some success. 
                 								
								

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